Data Details
Disease Name idiopathic pulmonary fibrosis
Tissue lung
Database GEO
Data ID GSE15197
Data Type expression
Platform GPL6480
Species homo sapiens
Sample Number 39
PubMed ID 20081107
Title RNA Expression Profiling of Lung Tissue Identifies Mutually Distinct Molecular Signatures in PAH and PH Secondary to IPF
Data Description "Fresh frozen lung tissue specimens from PAH subjects (n=18), IPF subjects with secondary PH (n=8), and normal controls (n=13) were obtained from the University of Pittsburgh Health Sciences Tissue Bank following approval by the University of Pittsburgh Institutional Review Board.Total RNA was isolated from lung tissue using Trizol (Invitrogen) and purified using the RNeasy Mini kit (Qiagen). RNA quantity and quality was assessed using Agilent Nanodrop and Agilent 2100 Bioanalyzer, respectively. 500 Ng of total RNA meeting was Cy3 labeled using the Agilent Low RNA Input Linear Amplification Kit PLUS (One-Color). After purification and fragmentation, aliquots of each sample were hybridized to an Agilent Whole Human Genome 4 X 44K microarray, sequentially washed, and scanned on an Agilent DNA microarray scanner."
Keyword pulmonary fibrosis non-coding RNA
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FDRdb: Fibrosis Disease-associated RNAnome database

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